3-D printers can produce gun parts, aircraft wings, food and a lot more, but this new 3-D printed product may be the craziest thing yet: human embryonic stem cells. Using stem cells as the "ink" in a 3-D printer, researchers in Scotland hope to eventually build 3-D printed organs and tissues. A team at Heriot-Watt University used a specially designed valve-based technique to deposit whole, live cells onto a surface in a specific pattern.
This article originally appeared at Popular Science.
The cells were floating in a "bio-ink," to use the terminology of the researchers who developed this technique. They were able to squeeze out tiny droplets, containing five cells or fewer per droplet, in a variety of shapes and sizes. To produce clumps of cells, the team printed out cells first and then overlaid those with cell-free bio-ink, resulting in larger droplets or spheroids of cells. The cells would group together inside these spheroids. Spheroid size is key, because stem cells need certain conditions to work properly. This is why very precisely controlled 3-D printing could be so valuable for stem cell research.
After being squeezed out of a thin valve, the cells were still alive and viable, and able to transform into any other cell in the body, the researchers say. It’s the first time anyone has printed human embyronic stem cells, said lead researcher Will Wenmiao Shu, a professor at Heriot-Watt. But ... why?
Eventually, they could be used to print out new tissues, or as filler inside existing organs, which would be regenerated. It could even serve to limit animal testing for new drug compounds, allowing them to be tested on actual human tissue, said Jason King, business development manager at Roslin Cellab, one of the research partners. "In the longer term, [it could] provide organs for transplant on demand, without the need for donation and without the problems of immune suppression and potential organ rejection," he said in a statement.
The team took stem cells from an embryonic kidney and from a well-studied embryonic cell line, and grew them in culture. They had to build a custom reservoir — let’s call it an inkwell — to safely house the delicate cells, and then they added some large-diameter nozzles. A pressurized air supply pumps the cells from the inkwell into the valves, which contain pressurized nozzles on the end. The team could control the amount of cells dispensed by changing any of the factors, including the pneumatic pressure, nozzle diameter or length of time the nozzle stayed open.
At first the researchers printed droplets, but ultimately, they were so precise that they made cell spheroids in a variety of shapes and sizes, like the university logo above. One interesting wrinkle: The cells also formed spheroids in the inkwells. More work needs to be done to explain that.
The researchers also took several steps to make sure the cells survived the printing process. Examining the results of several experiments, they found 99 percent of the cells were still viable after running through the valve-based printer.
In partnership with Roslin Cellab, the university’s biomedical engineering group came up with a valve-based printing technique that produces highly-viable, uniformly-sized droplets of stem cells. Most importantly, though, the cells maintain their pluripotency — the ability to differentiate into every adult human cell type. While the prospect of lab-grown custom organs is incredibly exciting, the group is careful to temper its outlook, noting that any such application would be "in the longer term."
Secret underground tunnels of ancient Mesopotamian cult revealed under Ani ruins 2014 09 01
For the first time in history, the academic world is paying attention to the spectacular underground world of Ani, a 5,000-year-old Armenian city located on the Turkish-Armenian border. Hurriyet Daily News reports that scientists, academics, and researchers have just met at a symposium in Kars titled ‘Underground Secrets of Ani’ to discuss the city’s underground world mentioned in ancient ...
A Government Vision Of The Future That Isn’t That Great 2014 09 01 Here’s a report by the UK Ministry of Defense, a document that they’re not hiding - it’s not classified. In fact, they WANT you to read it: the Global Strategic Trends 2045. For your convenience, they’ve even produced a handy video about their dire predictions:
They present a warning call for how things are going to be bad in the future. ...
Bad Memories Turned to Happy Ones in Mice Brains 2014 09 01 Memories are often associated with emotions, and these feelings can change through new experiences and over time. Now, using light, scientists have been able to manipulate mice brain cells and turn the animals’ fearful memories into happy ones, according to a new study.
Memories are encoded in groups of neurons that are activated together or in specific patterns, but it is ...
CIA MKULTRA: they intended to use drugs for “everything” 2014 09 01
Drugs to transform individuals…and even, by implication, society.
Drug research going far beyond the usual brief descriptions of MKULTRA.
The intention is there, in the record.
A CIA document was included in the transcript of the 1977 US Senate Hearings on MKULTRA, the CIA’s mind-control program.
The document is found in Appendix C, starting on page 166. It’s simply labeled “Draft,” dated 5 May ...
Harmful Compound Found in Many BPA-Free Bottles 2014 09 01 A chemical found in many products labeled “BPA free” may produce many of the same health problems as the original chemical, including hyperactivity and cardiac arrhythmia, according to a pair of studies presented recently at the joint meeting of the International Society of Endocrinology and the Endocrine Society in Chicago.
“BPS, termed the safe alternative to BPA, may be equally as ...