A 3D Printer That Generates Human Embryonic Stem Cells
2013-02-07 0:00

By Rebecca Boyle | io9.com


3-D printers can produce gun parts, aircraft wings, food and a lot more, but this new 3-D printed product may be the craziest thing yet: human embryonic stem cells. Using stem cells as the "ink" in a 3-D printer, researchers in Scotland hope to eventually build 3-D printed organs and tissues. A team at Heriot-Watt University used a specially designed valve-based technique to deposit whole, live cells onto a surface in a specific pattern.

This article originally appeared at Popular Science.

The cells were floating in a "bio-ink," to use the terminology of the researchers who developed this technique. They were able to squeeze out tiny droplets, containing five cells or fewer per droplet, in a variety of shapes and sizes. To produce clumps of cells, the team printed out cells first and then overlaid those with cell-free bio-ink, resulting in larger droplets or spheroids of cells. The cells would group together inside these spheroids. Spheroid size is key, because stem cells need certain conditions to work properly. This is why very precisely controlled 3-D printing could be so valuable for stem cell research.

After being squeezed out of a thin valve, the cells were still alive and viable, and able to transform into any other cell in the body, the researchers say. It’s the first time anyone has printed human embyronic stem cells, said lead researcher Will Wenmiao Shu, a professor at Heriot-Watt. But ... why?

Eventually, they could be used to print out new tissues, or as filler inside existing organs, which would be regenerated. It could even serve to limit animal testing for new drug compounds, allowing them to be tested on actual human tissue, said Jason King, business development manager at Roslin Cellab, one of the research partners. "In the longer term, [it could] provide organs for transplant on demand, without the need for donation and without the problems of immune suppression and potential organ rejection," he said in a statement.

The team took stem cells from an embryonic kidney and from a well-studied embryonic cell line, and grew them in culture. They had to build a custom reservoir — let’s call it an inkwell — to safely house the delicate cells, and then they added some large-diameter nozzles. A pressurized air supply pumps the cells from the inkwell into the valves, which contain pressurized nozzles on the end. The team could control the amount of cells dispensed by changing any of the factors, including the pneumatic pressure, nozzle diameter or length of time the nozzle stayed open.

At first the researchers printed droplets, but ultimately, they were so precise that they made cell spheroids in a variety of shapes and sizes, like the university logo above. One interesting wrinkle: The cells also formed spheroids in the inkwells. More work needs to be done to explain that.

The researchers also took several steps to make sure the cells survived the printing process. Examining the results of several experiments, they found 99 percent of the cells were still viable after running through the valve-based printer.

[...]

Read the full article at: io9.com





In partnership with Roslin Cellab, the university’s biomedical engineering group came up with a valve-based printing technique that produces highly-viable, uniformly-sized droplets of stem cells. Most importantly, though, the cells maintain their pluripotency — the ability to differentiate into every adult human cell type. While the prospect of lab-grown custom organs is incredibly exciting, the group is careful to temper its outlook, noting that any such application would be "in the longer term."
Source: TheVerge.com



Related Articles
Bioengineers ’3D Print’ Living Human Embryonic Stem Cells for First Time
This Child Has A Robot Hand Made With 3D Printers
Long-Gone Mollusk Comes to Life with 3D Printer
3D printer creates physical model of fetus for expecting parents
Artificial blood vessels created on a 3D printer


Latest News from our Front Page

CDC official called Obama 'Marxist,' 'amateur' over border surge...
2015-07-08 0:28
Following the influx of illegal immigrant minors from Central America, an official at the federal agency charged with protecting public health describes Barack Obama as "the worst pres we have ever had," an “amateur" and "Marxist," according to internal emails obtained by Judicial Watch. JW got the records as part of an investigation into the Center for Disease Control’s (CDC) activation ...
Obama Administration Likely to Block New Redskins Stadium
2015-07-07 23:03
The Obama administration will likely block Washington, D.C., authorities from building a new stadium for the NFL’s Washington Redskins because of objections to the team’s name. The National Park Service (NPS) owns the land under the 54-year-old Robert F. Kennedy Memorial Stadium, a venue two miles east of the Capitol that hosted the Redskins from 1961 to 1996. Some city leaders ...
Safe spaces, white tears and getting kicked out of a women's group - by two men
2015-07-07 21:37
Lately I've been kicked out of a couple of groups I belong to, for daring to question received wisdom or refusing to go along with the rules of identity politics. For instance, I was thrown out my university women's group -- by two men! -- for questioning an article on Jezebel about "cultural appropriation." They explained that the group was supposed to ...
Ghost rider in the sky: Scientists use lasers to project movie onto clouds
2015-07-07 20:08
A green ghost rider appeared in the sky over the British city of Nottingham when scientists started testing a newly developed projecting device which allows the beaming of moving images directly onto clouds for the first time ever. The image of a galloping horse rider was projected onto the clouds from a distance of 50 meters by a special laser-based projection ...
China’s stock market is crashing, and the Chinese are trying to do the exact same thing America did in 1929
2015-07-07 19:36
‘While European attention is focused on Greece, China is having a serious market meltdown. After exploding earlier in the year because of deregulation, China’s benchmark Shanghai Composite has collapsed a crazy 29% since the highs of early June. China’s other stock markets have had similarly steep falls. Bloomberg notes that the crisis is closely mirroring the 1929 Wall Street crash, which led ...
More News »