3-D printers can produce gun parts, aircraft wings, food and a lot more, but this new 3-D printed product may be the craziest thing yet: human embryonic stem cells. Using stem cells as the "ink" in a 3-D printer, researchers in Scotland hope to eventually build 3-D printed organs and tissues. A team at Heriot-Watt University used a specially designed valve-based technique to deposit whole, live cells onto a surface in a specific pattern.
This article originally appeared at Popular Science.
The cells were floating in a "bio-ink," to use the terminology of the researchers who developed this technique. They were able to squeeze out tiny droplets, containing five cells or fewer per droplet, in a variety of shapes and sizes. To produce clumps of cells, the team printed out cells first and then overlaid those with cell-free bio-ink, resulting in larger droplets or spheroids of cells. The cells would group together inside these spheroids. Spheroid size is key, because stem cells need certain conditions to work properly. This is why very precisely controlled 3-D printing could be so valuable for stem cell research.
After being squeezed out of a thin valve, the cells were still alive and viable, and able to transform into any other cell in the body, the researchers say. It’s the first time anyone has printed human embyronic stem cells, said lead researcher Will Wenmiao Shu, a professor at Heriot-Watt. But ... why?
Eventually, they could be used to print out new tissues, or as filler inside existing organs, which would be regenerated. It could even serve to limit animal testing for new drug compounds, allowing them to be tested on actual human tissue, said Jason King, business development manager at Roslin Cellab, one of the research partners. "In the longer term, [it could] provide organs for transplant on demand, without the need for donation and without the problems of immune suppression and potential organ rejection," he said in a statement.
The team took stem cells from an embryonic kidney and from a well-studied embryonic cell line, and grew them in culture. They had to build a custom reservoir — let’s call it an inkwell — to safely house the delicate cells, and then they added some large-diameter nozzles. A pressurized air supply pumps the cells from the inkwell into the valves, which contain pressurized nozzles on the end. The team could control the amount of cells dispensed by changing any of the factors, including the pneumatic pressure, nozzle diameter or length of time the nozzle stayed open.
At first the researchers printed droplets, but ultimately, they were so precise that they made cell spheroids in a variety of shapes and sizes, like the university logo above. One interesting wrinkle: The cells also formed spheroids in the inkwells. More work needs to be done to explain that.
The researchers also took several steps to make sure the cells survived the printing process. Examining the results of several experiments, they found 99 percent of the cells were still viable after running through the valve-based printer.
In partnership with Roslin Cellab, the university’s biomedical engineering group came up with a valve-based printing technique that produces highly-viable, uniformly-sized droplets of stem cells. Most importantly, though, the cells maintain their pluripotency — the ability to differentiate into every adult human cell type. While the prospect of lab-grown custom organs is incredibly exciting, the group is careful to temper its outlook, noting that any such application would be "in the longer term."
Soldier Beheaded in Broad Daylight Machete Attack 2013 05 23 Woolwich attack: terrorist proclaimed ’an eye for an eye’ after attack
A British soldier has been butchered on a busy London street by two Islamist terrorists, one of whom proclaimed afterwards: “An eye for an eye and a tooth for a tooth.”
In the first terrorist murder on the British mainland since the 7/7 suicide bombings of 2005, the men attempted ...
Ciudad Blanca Found? The lost city in Honduras 2013 05 23 Explorers have been searching on foot for Honduras’s mythical city for generations. Now, they seem to have found it from a tiny Cessna airplane, aided by million-dollar technology.
Is the fabled lost city of Honduras hiding beneath the dense jungle canopy?
The Mosquitia rain forests of Honduras and Nicaragua are, to put it mildly, thick jungle. As one travel guide notes, "While ...
Cheetah-bot races into your post-apocalyptic nightmares 2013 05 23 An ongoing robotics project at MIT aiming to recreate the gait of a cheetah is sharing a new video showing off the latest progress. There’s a long way to go before anyone would call it catlike, but it’s impressive nevertheless.
The Biomimetic Robotics Lab at MIT is attempting to create things much like those being made by the more well-known ...
When an Army of Artists Fooled Hitler 2013 05 23 Shortly after the D-Day invasion on June 6, 1944, two Frenchmen on bicycles managed to cross the perimeter of the United States Army’s 23rd Headquarters Special Troops and what they saw astounded them. Four American soldiers had picked up a 40-ton Sherman tank and were turning it in place.
Soldier Arthur Shilstone says, “They looked at me, and they were ...